Research Article
Involvement of the Bacillus SecYEG Pathway in Biosurfactant Production and Biofilm Formation
Figure 3
Biofilm formation by Bacillus isolates. (a) Black colony after 18 h of incubation, (b) diffusion on Congo Red agar after 24 h of incubation, (c) strong diffusion after 36 h of incubation, (d) complete diffusion after 48 h of incubation, (e) crystal violet assay. C+: B. subtilis strain GL48 used as a control in this study and C-: E. coli BL21 used as a negative control, (f) LB medium supplemented with Congo Red, NM2: isolates.
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