International Journal of Microbiology

Research Article

Involvement of the Bacillus SecYEG Pathway in Biosurfactant Production and Biofilm Formation

Table 2

Constructions, primers, and restriction enzymes used in this work.
Name of primersPrimer sequences (5′----3′)Restriction enzymesSize of PCR fragment (bp)CharacteristicsGiven nameReferences
SecAs55′AGG​ATC​CCT​TGG​AAT​TTT​AAA​TAA​AAT​GTT​TGA​TCC-3′BamHI2523pGEX6P-1-SecApKIKO3This study
SecAAs55′TGTC​GACCTA​TTC​AGT​ACG​GCC​GCA​GCA​AT-3′SalI
TasASF TasAasF5′TAG​GAT​CCG​GTA​TGA​AAA​AGA​AAT​TGA​G-3′ 5′TGT​CGA​CTT​AAT​TTT​TAT​CCT​CGC​TAT​GCG​CTT​TTT​C3′BamHI–SalI786pGEX4T1-TasApKIKO4This study
TapA-sF TapA-asF5′TAG​GAT​CCT​TTC​GAT​TGT​TTC​ACA​ATC​AGC-3′ 5′TGT​CGA​CTT​ACT​GAT​CAG​CTT​CAT​TGC​TTT​TTT​C-3′BamHI–SalI762pGEX4T1-TapApKIKO5This study
AprE1 AprE25′TCT​CGA​GGT​GAG​AAG​CAA​AAA​ATT​GTG​GAT​CAG-3′ 5′AGG​TAC​CTT​GTG​CAG​CTG​CTT​GTA​CGT​TGA​T-3′Xho1–KpnI1146pBAD-aprE-HispKIKO9This study
AmyE-s AmyE-As5′TCT​CGA​GAT​GTT​TGC​AAA​ACG​ATT​CAA​AAC​CTC-3′ 5′AGG​TAC​CAT​GGG​GAA​GAG​AAC​CGC​TTA​AGC​CCG-3′Xho1–KpnI659pBAD-amyE-HispKIK10This study
TasA-TasA-As s5′TCT​CGA​GAT​GGG​TAT​GAA​AAA​GAA​ATT​GAG-3′ 5′AGG​TAC​CAT​TTT​TAT​CCT​CGC​TAT​GCG​CTT​TTT​C-3′Xho1–KpnI786pBAD-TasA-HispKIKO15This study
TapA-S TapA-aS5′TCT​CGA​GAT​GTT​TCG​ATT​GTT​TCA​CAA​TCA​GC-3′ 5′AGG​TAC​CCT​GAT​CAG​CTT​CAT​TGC​TTT​TTT​C-3′Xho1–KpnI762pBAD-TapA-HispKIKO20This study