The Scientific World Journal

Research Article

Molecular Detection and Phylogenetic Analysis of the alkB Gene in Klebsiella oxytoca Strains Isolated from the Gut of Tenebrio molitor

Table 1

DNA sequences that were used to design 16S ribosomal RNA gene-specific primers for the 3 bacterial strain.
Strain nameSequence
Klebsiella oxytoca ATCC 13182TCGTGGGGGGGCCGWGGCCCTTAMACATGCAAGCTCKAAACGGTARA-AAAAGGGGGGGAAGSTCTCGGGTGACGAGTGTTARRTAAAGGARAAGW-AAGTCTGGGAAACTGCCCGATGGARGGGGATAACTACTGGAAATTTTT-ARWTAAKACCGCATACGTCGCAAGACCAAAGAKGGGGACCTTMRGGCC-TCTTGCCATCGGATGTGCCCRATGGGATTAGCTTGTAGTGAGGTA-ACGGCTCACCTAGCGACGATCCCTAGCTGGTCTGAGAGGATGAC-CARCCACACTGGAACTGAGAC-ACGGTCCARACTCCTAGKGGAGGGCAGCRKTGGGGAATATTGCACAA-TGGGGCGRAAGCCTTGATGCAKCCATTGCC-GCCGTGTATGAAGAAGGCCTTCRGGGTTTGWAAARTT-WCTTTTCASCCGGKGAGGAAAAGGGAATTGMAGGTTTAATAAC-CCTTTTTTCCTTTRACGTTTTACCCCACCARAAAAAAGSC-WCCCKGGTTAAAYTTTYCC
Klebsiella oxytoca JCM 1665ACKGGGCGGCARGCCGTACACATGCAAGTCGAACGGTAGCATAR-ARAGMTTGCTCTCGGGTGACGAGTGGCGGACGGGTGAGTAATGTCT-GGGAAACTGCCCGATGGAGGGGGATAACTAC-TGGAAACGGTAGCTAATACCGCATAA-CGTCGCAAGACCAAAGAGGGGGACCTTMGGGCCTCTTGCCATCGGATG-TGCCCAGATGGGATTAGCTTGTAGGTGAGGTAACGGCTC-ACCTAGGCGACGATCCCTAGCTGGTCTGAGAGG-ATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGA-GGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCA-TGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAG-CGGGGAGGAAGGG AGT​GAG​GTT​AAT​AAC​CTT​ATT​CAT​TGA​CGT​TAC​CC-GCAG​AAG​AAG​CAC​CGG​CTA​ACT​CCG​TGC​CAG​CAG​CCG​CGG​TAT​TACGG-A​GGG​TGC​AGC​GTT​ATC​GGA​ATT​ACT​GGG​GCG​TAA​AG
Klebsiella oxytoca NBRC 102593CAAYGGGTKKGGGGGGGYKGGCCGTACACATGSCWWGGTCGMACGGTAGTAGTRAAGAAGCTTGCTCTCGGGTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCCGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTMGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTTGTAGGTGAGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTGAGGTTAATAACCTTATTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGGGTGCAAAGCCGTTTAAATCC GGGGAAT